Endometrial Derived Stem Cells and Their Methods of Use

A method of making an insulin-producing cell derived from an endometrial stromal stem cell (ESSC), the method comprising the steps of:

• a. contacting at least one ESSC with a first cell culture medium comprising 20-30 mmol/l glucose, 5-15% FBS and 10−5-10−7 mol/l retinoic acid, and incubating the at least one cell for about 12-36 hours;
• then
• b. contacting the at least one ESSC with a second cell culture medium comprising 20-30 mmol/l glucose and 5-15% FBS and incubating the at least one ESSC for about 1-4 days;
• then
• c. contacting the at least one ESSC with ECM gel from Engelbreth-Holm-Swarm murine sarcoma and a third cell culture medium comprising 3-10 mmol/l glucose, 5-15% FBS, 3-30 mmol/l nicotinamide, 5-50 ng/ml epidermal growth factor, 5-500 ng/ml of FGF-10, and 50-600 nmol/l (−)-indolactam V and incubating the at least one ESSC for about 5-15 days;
• then
• d. contacting the at least one ESSC with a fourth cell culture medium comprising 5-15% FBS, 1-100 nmol/l exendin-4, and 5-500 ng/ml Activin A and incubating the at least one ESSC for about 3-15 days;
• thereby deriving an insulin-producing cell from an ESSC.