Compositions and method for manipulating pim-1 activity in circulatory system cells
AssigneeThe Regents of the University of California
InventorsMark A. SUSSMAN, John A. MURASKI
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Abstract
The invention provides compositions (e.g., pharmaceutical compositions) comprising nucleic acids encoding the serine/threonine kinase PIM-1, and methods for making and using them; including methods for inducing cellular proliferation, and protecting cardiac cells from hypoxia and cellular apoptosis. The invention provides compositions (e.g., pharmaceutical compositions) comprising nucleic acids encoding PIM-1, and methods for enhancing the regenerative potential of stem cells in the heart.
Claims17
A method, comprising identifying a patient in need of enhanced PIM activity in a vascular system cell or tissue, and enhancing levels of PIM in the vascular system cell or tissue of the patient to alter a functional characteristic of cells in that tissue.
3-5. (canceled)
A composition, comprising a vascular system cell or a cell can differentiate into a vascular system cell, wherein the cell comprises a PIM-encoding polynucleotide sequence operably linked to a non-PIM promoter.
(canceled)
A pharmaceutical composition formulated for administration to a heart muscle comprising:
- a Pim-1 encoding nucleic acid operably linked to a promoter and inserted in a viral vector.
11-12. (canceled)
A method for inducing expression of a PIM-1 nucleic acid in a cardiac or vascular cell or tissue ex vivo, comprising:
- (a) providing a Pim-1 encoding nucleic acid;
- and inserting the Pim-1 encoding nucleic acid into the cardiac or vascular cell or tissue;
- (ii) providing a cell expressing and/or secreting a Pim-1 kinase;
- (iii) administering Pim-1 kinase or a Pim-1 expressing nucleic acid to the cardiac or vascular cell, tissue or organ;
- or, (iv) providing a compound that induces or upregulates PIM-1 nucleic acid or a Pim-1 kinase activity in a cardiac or vascular cell, tissue or organ;
- and
- (b)
- implanting the cardiac or vascular cell or tissue into an individual in need thereof.
(canceled)
16-17. (canceled)
Dependencies are inferred from the claim text.