Commensal Bacteria As Signal Mediators Within A Mammalian Host
AssigneeCornell University
InventorsJohn C. March, Franklin Faping Duan
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Abstract
Genetically engineered cells and microorganisms are provided for preventing or ameliorating diseases through genetically engineered quorum signaling. Therapeutic methods for using the cells and microorganisms to prevent or ameliorate diseases are also provided. The genetically engineered cells or microorganisms can be engineered to express a signal and used to interrupt the signaling-dependent virulence of an invading pathogen. The cells or microorganisms can be used to provide signal-dependent expression of a desirable gene in order to interrupt, prevent, and/or ameliorate a disease of mammals, such as parasitic diseases, infectious diseases, autoimmune diseases and genetic disorders.
Claims13
A recombinant enteric commensal bacterium comprising a recombinant nucleic acid encoding a signal that comprises a mammalian insulin secretion-stimulating peptide, wherein:
- the recombinant enteric commensal bacterium is selected from the group consisting of:
- gram-negative bacterium and gram-positive bacterium,
- the recombinant nucleic acid encoding the signal is under the control of a promoter, and
- the signal stimulates glucose-responsive insulin production in a mammalian subject.
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A method treating diabetes in a mammalian host comprising:
- administering to the gastrointestinal tract of mammalian host a recombinant enteric commensal bacterium comprising a recombinant nucleic acid encoding a signal that comprises a mammalian insulin secretion-stimulating peptide, wherein:
- the recombinant enteric commensal bacterium is selected from the group consisting of:
- gram-negative bacterium and gram-positive bacterium,
- the recombinant nucleic acid encoding the signal is under the control of a promoter, and
- the signal stimulates glucose-responsive insulin production in a mammalian subject.
An isolated recombinant cell comprising a recombinant nucleic acid encoding a signal, wherein:
- the cell is derived from a first organism that is a microorganism,
- the signal is capable of being expressed by the cell,
- the signal is secreted by the cell and secretion by the cell is controlled by an environmental stimulus that is secreted by an invading pathogen and the signal inhibits or disrupts the pathogenicity or virulence of the invading pathogen.,
- the signal regulates signal-dependent expression of a target nucleic acid that controls pathogenesis or virulence of a pathogen.
Dependencies are inferred from the claim text.