Methods and systems for background subtraction in an image
AssigneeLife Technologies Corporation
InventorsFrancis T. Cheng, Thomas Wessel
Life of this patent
Estimating the term…
Abstract
A method for improving image quality is provided. The method includes receiving image data of a substrate, wherein the image data is generated by imaging the substrate, and an image is generated from the image data. The method further includes generating a background representation from a background noise portion of the image, wherein the background portion includes signal information undesired for further processing and generating a background subtracted image by subtracting the background rep resentation from the image. In this way, a separate background image is not needed to subtract the background from the image including the regions-of-interest to improve image quality.
Claims29
A method for improving image quality, the method comprising:
- receiving image data of a substrate, wherein the image data is generated by imaging the substrate, and an image is generated from the image data;
- generating a background representation from a background noise portion of the image, wherein the background portion includes signal information undesired for further processing;
- generating a background subtracted image by subtracting the background representation from the image.
A computer-implemented method of determining a position of a substrate, the method comprising:
- receiving, by a processor, image data of a substrate, wherein the image data is generated by imaging the substrate;
- generating, by the processor, projection data based on the image data;
- evaluating, by the processor, the projection data for a known landmark pattern of the substrate, wherein the known landmark pattern is stored in a memory;
- and
- determining, by the processor, positions of the known landmark pattern of the substrate in the image data based on the evaluating.
A computer-readable storage medium encoded with instructions, executable by a processor, the instructions comprising instructions for:
- receiving image data of a substrate, wherein the image data is generated by imaging the substrate;
- generating projection data based on the image data;
- evaluating the projection data for a known landmark pattern of the substrate, wherein the known landmark pattern is stored in a memory;
- and
- determining positions of the known landmark pattern of the substrate in the image data based on the evaluating.
A system for visualizing a plurality of data plots, the system comprising:
- a processor;
- and
- a memory encoded with instructions for:
- receiving image data of a substrate, wherein the image data is generated by imaging the substrate;
- generating projection data based on the image data;
- evaluating the projection data for a known landmark pattern of the substrate, wherein the known landmark pattern is stored in the memory;
- and
- determining positions of the known landmark pattern of the substrate in the image data based on the evaluating.
A method for improving image quality, the method comprising:
- determining a first portion of a pedestal for a first area in an image, wherein the pedestal includes signal information undesired for further processing;
- interpolating a second pedestal portion for a second area in the image based on the first portion of the pedestal, wherein the second area does not include regions-of-interest;
- generating a pedestal representation by combining first and second portion of the pedestal;
- applying a smoothing function over the first and second portion of the pedestal;
- and
- subtracting the pedestal representation from the image to generate a background subtracted image.
Dependencies are inferred from the claim text.