Method for Varying the Scanning Field of A Laser Scanning Microscope
AssigneeCarl-Zeiss-Promenade 10
InventorsDaniel Schwedt, Tiemo Anhut, Tobias Kaufhold
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Abstract
Disclosed is a method for varying the size of the scanning field of a multifocal laser scanning microscope, said scanning field being scanned in X columns and Y lines, and n laser spots being arranged at a distance d from one another in the scanning field along the slow scanning axis in the sample plane, the distance between the scanned lines in the sample plane being a=d/K, where K ε N, the size of the scanning field being varied by varying K. After scanning K lines, a vertical skip is made, e.g. a skip of (n−1)×K+1 lines in the scanning direction or (n+1)×K−1 lines against the scanning direction until at least Y lines have been scanned.
Claims13
Method of varying the size of the scanning field of a multifocal laser scanning microscope, which is scanned in X columns and Y lines, characterised in that n laser spots are disposed in the scanning field along the slow scanning axis in the sample plane in each case with a spacing d relative to one another, and the spacing of the scanned lines in the sample plane is a=d/K with K ε N, wherein the variation in the size of the scanning field takes place by variation of K, and after the scanning of K lines a vertical skip takes place, for example through a skip by (n−1)×K+1 lines in the scanning direction or (n+1)×K−1 lines against the scanning direction, until at least Y lines have been scanned.
Dependencies are inferred from the claim text.