US 2014/0231619 A1 · OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS USING SINGLE LIGHT-EMITTING PARTICLE DETECTION

Abstract

There is provided a way of enabling the discrimination or identification of the kind of a light-emitting particle corresponding to each pulse form signal in the scanning molecule counting method using the optical measurement by the confocal or multiphoton microscope. In the inventive technique, the position of a light detection region in a sample solution periodically along a predetermined route is moved in measuring the light intensity from the light detection region; and a signal of light from a light-emitting particle is detected individually. Then, an index value indicating a translational diffusional characteristic of one light-emitting particle in a plane perpendicular to the moving direction of the light detection region is determined based upon intensity values of signals of light of the same light-emitting particle for identifying a light-emitting particle.

Claims12

Dependent claims

An optical analysis device which detects light from a light-emitting particle dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, comprising:

a light detection region mover which periodically moves a position of a light detection region of the optical system along a predetermined route in the sample solution;
a light detector which detects a light amount from the light detection region;
and
a signal processor which generates time series light intensity data of the light from the light detection region detected with the light detector during the moving of the position of the light detection region in the sample solution and detects a signal indicating light from a single light-emitting particle individually in the time series light intensity data;
wherein the signal processor determines an index value indicating a translational diffusional characteristic of the light-emitting particle in a plane perpendicular to a moving direction of the light detection region based upon an intensity value of a detected signal indicating light of one light-emitting particle and an intensity value within a time region separated from a generation time of the signal indicating light of the one light-emitting particle by a time corresponding to an integral multiple of a moving cycle of the position of the light detection region in the time series light intensity data.

2 more elements

An optical analysis method of detecting light from a light-emitting particle dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, comprising steps of:

(a) moving a position of a light detection region of the optical system in the sample solution periodically along a predetermined route;
(b) measuring a light intensity from the light detection region during the moving of the position of the light detection in the sample solution to generate light intensity data;
(c) detecting individually a signal indicating light from a single light-emitting particle in the light intensity data;
and
(d) determining an index value indicating a translational diffusional characteristic of one light-emitting particle in a plane perpendicular to a moving direction of the light detection region based upon an intensity value of a detected signal indicating light of the one light-emitting particle and an intensity value within a time region separated from a generation time of the signal indicating light of the one light-emitting particle by a time corresponding to an integral multiple of a moving cycle of the position of the light detection region in the time series light intensity data.

2 more elements

A computer readable storage device having a computer program product including programmed instructions for optical analysis of detecting light from a light-emitting particle dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, said programmed instructions causing a computer to perform steps comprising:

moving a position of a light detection region of the optical system in the sample solution periodically along a predetermined route;
measuring a light intensity from the light detection region during the moving of the position of the light detection region in the sample solution to generate light intensity data;
detecting individually a signal indicating light from a single light-emitting particle in the light intensity data;
and
determining an index value indicating a translational diffusional characteristic of one light-emitting particle in a plane perpendicular to a moving direction of the light detection region based upon an intensity value of a detected signal indicating light of the one light-emitting particle and an intensity value within a time region separated from a generation time of the signal indicating light of the one light-emitting particle by a time corresponding to an integral multiple of a moving cycle of the position of the light detection region in the time series light intensity data.

2 more elements

Dependencies are inferred from the claim text.

Optical analysis device, optical analysis method and computer program for optical analysis using single light-emitting particle detection

Life of this patent

Optical analysis device, optical analysis method and computer program for optical analysis using single light-emitting particle detection

Life of this patent

Abstract

Claims12