Modified plants with increased oil content
AssigneeBrookhaven Science Associates, LLC
InventorsJohn Shanklin, Carl Andre
Life of this patent
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Abstract
Methods and means are provided to increase the oil content of plants, particularly oleaginous plants by preventing feedback inhibition by 18:1-Coenzyme A or 18:1-Acyl Carrier Protein of the acetyl CoA-carboxylase enzyme in cells of these plants in various manners.
Claims39
A method to increase oil content in cells of a plant, comprising the step of preventing feedback inhibition by 18:1-Coenzyme A or 18:1-Acyl Carrier Protein of the plastidic acetyl CoA-carboxylase enzyme in said cells of said plant by expressing in said plant cells an acetyl CoA-carboxylase enzyme or subunit thereof from an organism that uses the 3-hydroxypropionate cycle for carbon fixation.
2-5. (canceled)
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The method according to 1, wherein said acetyl CoA-carboxylase enzyme or subunit thereof is any of SEQ ID Nos. 46-128.
15-26. (canceled)
A plant comprising in its plastids one or more ACCase variant enzymes or subunits thereof which are less sensitive to feedback inhibition by 18:1-Coenzyme A or 18:1-Acyl Carrier Protein than a wild-type acetyl CoA-carboxylase of said plant, wherein said acetyl CoA-carboxylase enzyme or subunit thereof is from an organism that uses the 3-hydroxypropionate cycle for carbon fixation.
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A chimeric DNA comprising the following operably linked DNA fragments
- a) a plant expressible promoter;
- b) one or more coding regions encoding one or more acetyl CoA-carboxylase subunits having an amino acid sequence of SEQ ID Nos. 2, 3, 5, 7, 9, 11, 13, 15, 17 or 19;
- or a coding region having 70% sequence identity with an amino acid sequence of SEQ ID Nos. 2, 3, 5, 7, 9, 11, 13, 15, 17 or 19 or any of SEQ ID Nos. 46-128 and having acetyl CoA carboxylase enzymatic activity;
- and optionally
- c) a transcription termination and/or polyadenylation region functional in plant cells.
45-49. (canceled)
Dependencies are inferred from the claim text.