Recombinant constructs and transgenic fluorescent ornamental fish therefrom
AssigneeYorktown Technologies, L.P.
InventorsAlan BLAKE, Richard CROCKETT, Jeffrey ESSNER, Perry HACKETT, Aidas NASEVICIUS
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Abstract
The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.
Claims22
-71. (canceled)
A method of producing a transgenic zebrafish having improved fluorescence comprising:
- (a) obtaining a transgenic, fluorescent zebrafish that exhibits a fluorescence and comprises:
- i) a first transgene encoding a fluorescent protein, wherein the first transgene is under the control of a ubiquitous fish promoter, and
- ii) a second transgene encoding the same fluorescent protein as in the first transgene, wherein the second transgene is under the control of a tissue-specific fish promoter;
- and
- (b) selecting transgenic fish exhibiting strong visible fluorescence over 75% to 100% of the body of said zebrafish, optionally excluding fins and eyes.
A transgenic zebrafish comprising a chromosomally integrated expression cassette encoding a DsRed2 gene, a ZsGreen1 gene, or a ZsYellow1 gene, wherein the zebrafish respectively comprise the Red zebrafish 1 transformation event, Green zebrafish 1 transformation event, or Yellow zebrafishl transformation event, sperm comprising the Red zebrafish 1 transformation event, the Green zebrafish 1 transformation event, and the Yellow zebrafish 1 transformation event having been deposited as ECACC accession nos. 06090403, 06090401, and 06090402, respectively.
Dependencies are inferred from the claim text.