Coregistering Images of Needle Biopsies Using Multiple Weighted Landmarks

A method comprising:

• defining a first middle path through a first image object of a first digital image of a first tissue slice;
• defining a second middle path through a second image object of a second digital image of a second tissue slice;
• defining first landmarks on the first middle path;
• defining second landmarks on the second middle path;
• associating individual first landmarks with individual second landmarks;
• coregistering a first pixel in the first image object with a second pixel in the second object using multiple first landmarks and multiple second landmarks;
• and
• displaying on a graphical user interface the first digital image in a first frame and the second digital image in a second frame, wherein the first pixel is centered in the first frame and the second pixel is centered in the second frame.

A method comprising:

• displaying a first digital image of a first tissue slice on a graphical user interface such that an area of the first digital image is enclosed by a frame;
• displaying a portion of a second digital image of a second tissue slice on the graphical user interface, wherein the first tissue slice and the second tissue slice are taken from a needle biopsy, wherein the displayed portion of the second digital image has a shape of the frame, and wherein the displayed portion of the second digital image corresponds to the area of the first digital image that is enclosed by the frame;
• initially coregistering a pixel that is centered in the displayed portion of the second digital image with an associated pixel of the first digital image that is centered in the frame using multiple second landmarks in the second digital image and multiple associated first landmarks in the first digital image;
• and
• shifting the displayed portion of the second digital image in response to a user shifting the first digital image relative to the frame, wherein the second digital image is shifted such that each coregistered pixel of the displayed portion of the second digital image is determined using first landmarks that are weighted based on their distance from the center of the frame.