Production of oil in vegetative tissues

A method, comprising,

• a) providing,
• i) a plant tissue, wherein said plant tissue comprises a RNAi vector construct, comprising a first promoter sequence in operable combination with an RNAi gene silencing cassette having a first fragment of a trigalactosyldiacylglycerol nucleic acid in its sense sequence orientation and a second fragment of a trigalactosyldiacylglycerol nucleic acid its antisense sequence orientation for reducing a trigalactosyldiacylglycerol protein, and
• ii) a double gene vector construct for a first gene comprising a RNAi silencing for reducing an ADP-glucose pyrophosphorylase protein and a second gene for expressing a heterologous oil regulating transcription factor nucleic acid sequence for increasing transcription factor protein, and
• b) transfecting said plant tissue with said double gene vector and growing tissue into a whole plant, wherein said plant has reduced trigalactosyldiacylglycerol protein, reduced AGPase protein and increased transcription factor protein for increased lipid in vegetative tissues of said plant.

A method for making a plant, comprising,

• a) providing,
• i) a plant tissue, wherein said plant tissue has reduced ADP-glucose pyrophosphorylase protein and increased expression of a heterologous oil regulating transcription factor protein,
• ii) a RNAi silencing construct comprising a first promoter sequence in operable combination with a first fragment of a trigalactosyldiacylglycerol nucleic acid sequence in its sense orientation and a second fragment of a trigalactosyldiacylglycerol nucleic acid sequence it's antisense orientation, and
• b) transfecting said RNAi vector construct into said plant tissue,
• c) growing said plant tissue into a plant, wherein said plant accumulates lipid in its vegetative tissue.

A method, comprising,

• a) providing,
• i) a first plant, wherein said plant has reduced ADP-glucose pyrophosphorylase protein and increased expression of a heterologous oil regulating transcription factor,
• ii) a second plant, wherein said plant has reduced trigalactosyldiacylglycerol protein, and
• b) breeding said first plant with said second plant for producing seed capable of growing into a third plant, and
• c) growing said seed into said third plant, wherein said third plant accumulates lipid in its vegetative tissue.