Viral clearance methods
AssigneeHuman Genome Sciences, Inc.
InventorOlga Galperina
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Abstract
The invention provides methods for separating a polypeptide of interest (such as an antibody) from a virus. In some embodiments, the methods involve eluting the polypeptide of interest from a Protein A resin with an elution buffer have a particular range of conductivity values that minimizes the amount of virus that co-elutes with the polypeptide of interest.
Claims22
A method for separating a polypeptide of interest from a virus, the method comprising applying an elution buffer having a conductivity between about 3.5 to about 9.5 mS/cm to a Protein A resin having a polypeptide of interest and a virus adsorbed to the resin, wherein the elution of the polypeptide of interest from the resin separates the polypeptide of interest from at least a portion of the virus.
A method for purifying a polypeptide of interest that is capable of binding a Protein A resin, the method comprising:
- a. applying a solution comprising the polypeptide of interest and a virus to the Protein A resin under conditions such that the polypeptide of interest binds to the Protein A resin;
- b. washing the resin with a wash buffer;
- and
- c. eluting the polypeptide of interest from the resin with an elution buffer having a conductivity between about 3.5 to about 9.5 mS/cm to provide a recovered composition.
A method, for purifying a polypeptide of interest that is capable of binding a Protein A resin, the method comprising;
- a. applying a solution comprising the polypeptide of interest and a virus to the Protein A resin under conditions such that the polypeptide of interest binds to the Protein A resin;
- b. washing the resin with a wash buffer;
- c. eluting the polypeptide of interest from the resin with a first elution buffer to provide a recovered composition;
- d. measuring the amount of virus in the recovered composition;
- and
- e. if the amount of virus in step (d) is greater than desired, the repeating steps (a) to (c) with a second elution buffer with a higher conductivity than the first elution buffer used in step (c).
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Dependencies are inferred from the claim text.