Separation of acetylated proteins from unacetylated proteins
AssigneesTanvex Biologics Corp., La Jolla Biologics, Inc.
InventorsKuo-Ming YU, Judy Huei-chuan CHOU, Jennifer Renee HOPP
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Abstract
The invention relates to a process for separating acetylated proteins from unacetylated proteins. In particular, the invention relates to a process of using multimodal chromatography to separate acetylated proteins from unacetylated proteins. In the prior art, multimodal chromatography is used to isolate antibodies. The invention found that multimodal chromatography can be used in the separation of acetylated proteins from unacetylated proteins.
Claims24
A process for separating acetylated proteins from unacetylated proteins, comprising applying an impure preparation comprising acetylated and unacetylated proteins to a multimodal chromatography support and conducting elution with a change in pH.
A process of purifying recombinant interferons produced from an expression system to obtain unacetylated forms thereof, comprising the steps of:
- (a) subjecting solubilized and refolded interferons produced from an expression system to hydrophobic interaction chromatography, wherein the interferons comprise acetylated forms and unacetylated forms;
- and
- (b) subjecting the interferons from step (a) to the process for separating acetylated proteins from unacetylated proteins mentioned herein so that the unacetylated form interferons can be obtained.
Dependencies are inferred from the claim text.