Assays and biomarkers for lrrk2
AssigneeGenentech, Inc.
InventorsDaisy Bustos, Donald Kirkpatrick, Tracy Kleinheinz, John Moffat, Zejuan Sheng, Shuo Zhang, Haitao Zhu
Life of this patent
Estimating the term…
Abstract
Biomarkers for screening subjects for Parkinson's disease and providing companion diagnostic tools for therapies using LRRK2 modulators, and assays for screening compounds and compositions for modulation of LRRK2 activity.
Claims16
A method for identifying a patient or subject having Parkinson's disease or at risk of developing Parkinson's disease, the method comprising determining the level of S1292 autophosphorylation in said subject, wherein an elevated level correlates with having Parkinson's disease or risk of developing Parkinson's disease.
A method for predicting the sensitivity of a subject to a LRRK2 modulator, or efficacy of a LRRK2 modulator for treating Parkinson's disease, the method comprising determining the level of S1292 autophosphorylation in said subject, whereby elevated levels of S1292 autophosphorylation correlate with efficacy of said inhibitor for treating said disease.
a method for identifying subjects having Parkinson's disease or that are at risk of developing Parkinson's disease, the method comprising:
- obtaining or acquiring a LRRK2-containing sample from a subject;
- measuring, monitoring or otherwise detecting the amount or level of S1292 autophosphorylation in the subject sample;
- and
- comparing the amount or level of S1292 autophosphorylation in the subject sample with the amount or level of S1292 autophosphorylation in one or more control samples.
A method for determining subject response to therapy involving a LRRK2 modulator, the method comprising:
- obtaining or acquiring a first LRRK2-containing sample from a subject;
- administering a LRRK2 modulator to the subject;
- obtaining or acquiring a second LRRK2-containing sample from the subject after administering the LRRK2 modulator to the subject;
- measuring, monitoring or otherwise detecting the amount or level of S1292 autophosphorylation in the first subject sample;
- measuring, monitoring or otherwise detecting the amount or level of S1292 autophosphorylation in the second subject sample;
- and
- comparing the amount or level of S1292 autophosphorylation in the first subject sample with the amount or level of S1292 autophosphorylation in the second subject sample.
An assay for screening compounds and compositions for inhibition of LRRK2 activity, the method comprising:
- exposing LRRK2 or a fragment thereof to a potential inhibitor compound;
- and
- detecting the level or amount of phosphorylation associated with S1292 on the LRRK2.
Dependencies are inferred from the claim text.