Antibiotics and methods for manufacturing the same
AssigneeThe Board of Trustees of the University of Illinois
InventorsDouglas A. Mitchell, Katie J. Molohon
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Abstract
The present invention generally relates to a novel highly discriminating antibiotic, plantazolicin, a plantazolicin-like compound and to pharmaceutical compositions comprising the same. Also provided are methods for producing and using plantazolicin. Due to its bactericidal activity against Bacillus anthracis, plantazolicin and plantazolicin-like compounds can be used in methods for treating and/or preventing anthrax infections.
Claims35
A plantazolicin-like compound having the structure:
- (X1)—(X2)5—(X3)2—(X4)5—(X5)n
- or a pharmaceutically acceptable salt or ester thereof,
- wherein
- X1 is
- R1 and R2 are each independently hydrogen or lower alkyl;
- each X2 is independently an azole;
- each X3 is independently a hydrophobic amino acid;
- each X4 is independently an azole or azoline;
- and
- each X5 is independently an amino acid, wherein n is 1 or 2.
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A method for identifying a plantazolicin-like protein, wherein the method comprises:
- identifying a bacterial amino acid sequence exhibiting at least 50% amino acid identity to a plantazolicin precursor peptide from Bacillus amyloliquefaciens FZB42;
- obtaining a post-translationally modified product of the bacterial amino acid sequence;
- and
- testing the post-translationally modified product of the bacterial amino acid sequence in a Bacillus anthracis growth inhibitory assay, wherein ability to inhibit the growth of Bacillus anthracis indicates that the bacterial amino acid sequence encodes a plantazolicin-like protein.
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Dependencies are inferred from the claim text.