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US 2014/0228245 A1Published application
Method for the spatial arrangement of sample fragments for amplification and immobilization for further derivatizations
AssigneeAlbert-Ludwigs-Universitaet Freiburg
InventorsJochen Hoffmann, Guenter Roth
Life of this patent
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Abstract
The invention relates to a method for performing a biochemical or chemical reaction for an isolated, spatially separated amplification of sample fragments during a simultaneous immobilization and spatial arrangement of the sample fragments and reaction products, the amplification products, on one or more suitable solid phases for subsequent derivatizations.
Claims32
A method for amplification of sample fragments, comprising:
- a. supplying a solid phase with a microstructure, wherein the microstructure subdivides the solid phase so that at least one, preferably several compartments are formed, and wherein capture molecules are attached to the solid phase,
- b. introducing at least one sample fragment into at least one compartment,
- c. introducing a reaction mixture for amplification in at least one compartment,
- d. separating and/or covering the individual compartments to obtain sealed compartments,
- e. amplifying each sample fragment independently in the corresponding sealed compartment to obtain an amplification products,
- f. wherein the amplification products generated by the amplifying in (e) are immobilized on the capture molecules.
3 more elements
A device comprising
- g) a solid phase with at least one, preferably several compartments, wherein capture molecules are bound to the surface of the solid phase, preferably to the compartments and
- h) a separation and/or cover.
A master array comprising
- i) a solid phase with at least one, preferably several compartments, wherein capture molecules are bound to the surface of the solid phase, preferably to the compartments, and wherein
- j) amplification products and/or derivatives of an amplification reaction are bound to the capture molecules.
(canceled)
(canceled)
A kit for amplification of sample fragments and/or for producing an array, comprising
- a solid phase with at least one, preferably several compartments, wherein capture molecules are bound to the surface of the solid phase, preferably to the compartments
- separation and/or cover and
- at least one reaction mixture.
Dependencies are inferred from the claim text.