Calibration Reagent and Method
AssigneePhadia AB
InventorsGöran Elfverson, Per Matsson, Mats Nystrand
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Abstract
The present invention relates to a method for calibrating a multiplex assay, comprising: adding a calibration reagent to a solid phase on which a plurality of capturing agents are immobilised, adding a detection molecule which has a capacity to bind to the calibration reagent, detecting bound detection molecule, thereby creating a calibration curve, wherein the calibration reagent comprises at least two different binding molecules, wherein each binding molecule has a capacity to bind specifically to a capturing agent immobilised on the solid phase and a capacity to bind to a detection molecule. Further provided is a multiplex assay system comprising such a calibration reagent.
Claims21
-20. (canceled)
A method for calibrating a multiplex assay, comprising:
- adding a calibration reagent to a solid phase on which a plurality of capturing agents are immobilised,
- adding a detection molecule which has a capacity to bind to the calibration reagent, and
- detecting bound detection molecule,
- thereby creating a calibration curve comprising a number of calibration points/intervals,
- wherein the calibration reagent comprises at least two different binding molecules, wherein each binding molecule has a capacity to bind specifically to a capturing agent immobilised on the solid phase and a capacity to bind to a detection molecule, and wherein at least two of the binding molecules have different specificities and are present at different concentrations in the calibration reagent, thereby representing different calibration points/intervals in the calibration curve.
A multiplex assay system for detection of a molecule of interest, such as (a) an immunoglobulin or (b) a peptide/protein biomarker present in a biological sample, comprising:
- a reaction vessel,
- a plurality of capturing agents immobilised on a solid phase,
- a detection molecule, such as (a) an anti-immunoglobulin detection molecule or (b) a detection molecule binding to a peptide/protein biomarker,
- a calibration reagent,
- a reaction buffer medium,
- wherein the calibration reagent comprises at least two different binding molecules, wherein each binding molecule has a capacity to bind specifically to a capturing agent immobilised on the solid phase and a capacity to bind to the detection molecule, and wherein at least two of the binding molecules have different specificities and are present at different concentrations in the calibration reagent.
The system of claim 10 wherein the molecule of interest is (i) an IgE antibody or (ii) an IgG antibody, or (iii) a peptide/protein biomarker for disease.
A calibration reagent comprising at least two different chimeric antibodies, wherein each chimeric antibody has the capacity to bind specifically to at least one of the allergen components listed in Table 2, and wherein at least two of the chimeric antibodies have different specificities and are present at different concentrations in the calibration reagent.
Dependencies are inferred from the claim text.