Target-recognition compositions comprising novel synthetic conjugates for trapping and diagnosis of a target molecule
OwnerNaomi B. Zakinventor
Life of this patent
Estimating the term…
Abstract
The invention relates to methods, kits and devices for sensitive and efficient trapping, removing, detecting or quantification of a target compound in or from a substance. More specifically, the invention encompasses the use of at least one target-recognition composition comprising at least one nucleic acid-based aptamer that specifically recognizes and binds the target compound, and at least one DNA dendrimer labeled with at least one trapping, partitioning or detectable moiety.
Claims22
A method for trapping, removing, detecting or quantitating, at least one target compound in or from a substance or any preparation or sample thereof, comprising the steps of:
- a. providing at least one target-recognition composition comprising at least one nucleic acid-based aptamer that specifically recognizes and binds said at least one target compound, or complex thereof, or any parts or fragments thereof, or any combinations thereof, and at least one nucleic acid based dendrimer labeled with at least one trapping, partitioning or detectable moiety;
- b. contacting said substance, or any preparation, sample, or an aliquot thereof with at least one of said target-recognition composition of (a) under conditions suitable for recognition and binding of said at least one target compound or any parts or fragments thereof, or any combinations thereof, by said at least one nucleic acid based aptamer to form at least one target-aptamer complex;
- and
- c. trapping or detecting said at least one trapping or detectable moiety in said labeled dendrimer comprised within said target-recognition composition, which dendrimer is bound to or conjugated with said at least one aptamer in said target-aptamer complex, thereby trapping, removing or determining the presence and the amount of said target compound in or from said substance.
A kit for trapping, removing, detecting, or quantitating, at least one target compound in/or from a substance or any preparation or sample thereof, comprising:
- a. at least one nucleic acid based aptamer that specifically recognizes and binds said at least one target compound, or complex thereof, or any parts or fragments thereof, or any combinations thereof;
- and
- b. at least one nucleic acid based dendrimer labeled with at least one trapping, partitioning or detectable moiety;
- c. optionally, instructions for carrying out the detection or trapping of said at least one detectable or trapping moiety in said labeled dendrimer.
A device for trapping, removing, detecting, or quantitating at least one target compound in/or from a substance or any preparation or sample thereof, comprising:
- a. at least one target-recognition composition comprising at least one nucleic acid based aptamer that specifically recognizes and binds said at least one target compound, or complex thereof, or any parts or fragments thereof, or any combinations thereof, and at least one nucleic acid based dendrimer labeled with at least one trapping, partitioning or detectable moiety;
- and
- b. a solid support suitable for the reception and transport of said sample.
Dependencies are inferred from the claim text.