Method to estimate age of individual based on epigenetic markers in biological sample
AssigneeThe Regents of the University of California
InventorsEric Vilain, Sven Bocklandt, Steve Horvath, Janet Sinsheimer
Life of this patent
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Abstract
The invention provides methods and materials that use observation of DNA characteristics to obtain information relating to the age of individuals. The instant disclosure identifies 88 sites in or near 80 genes for which the degree of cytosine methylation in epithelial and/or white blood cells is significantly correlated with age. In illustrative embodiments of the invention, cytosine methylation patterns the promoters of the EDARADD, TOMILI, and NPTX2 genes are used to predict the age of an individual with a high degree of accuracy.
Claims20
A method of obtaining information useful to determine an age of an individual, the method comprising the steps of:
- (a) obtaining a biological sample derived from an individual comprising genomic DNA from white blood cells or epithelial cells;
- and
- (b) observing a pattern of cytosine methylation of at least one genomic DNA sequence selected from the group consisting of Edaradd (SEQ ID NO:
- 1), TomL1 (SEQ ID NO:
- 2), NPTX2 (SEQ ID NO:
- 3) and ELN (SEQ ID NO:
- 4);
- so that information useful to determine the age of the individual is obtained.
A method of obtaining information useful to determine an age of an individual, the method comprising the steps of:
- (a) obtaining genomic DNA from white blood cells or epithelial cells derived from the individual;
- and
- (b) observing cytosine methylation of at least two CG loci in the genomic DNA selected from the group consisting of CG locus designation:
- cg27553955, cg12799895, cg21296230, cg04084157, cg25148589, cg17861230, cg03734874, cg18236477, cg27320127, cg07621046, cg14456683, cg21801378, cg00107187, cg05508084, cg07533148, cg03975694, cg19945840, cg00399483, cg06291867, cg24826867, cg06092815, cg19885761, cg24199834, cg04528819, cg13434842, cg02008154, cg25044651, cg02994956, cg20366906, cg27389185, cg14826456, cg12111714, cg12457773, cg11981599, cg00059225, cg06572160, cg08668790, cg12782180, cg20134215, cg13921352, cg13614181, cg23563234, cg00201234, cg21992250, cg00911351, cg19594666, cg15425280, cg06156376, cg19831077, cg27409364, cg15747595, cg02154186, cg20616414, cg10235817, cg16232126, cg24646414, cg17241310, cg10031651, cg23290344, cg06908778, cg02844545, cg14614211, cg25511429, cg15201635, cg19246110, cg06760035, cg13603171, cg13282837, cg20792062, cg02228185, cg13547237, cg09809672, cg01293143, cg05822532, cg07408456, cg08468689, cg27210390, cg01820374, cg17589341, cg19761273, cg03440846, cg08909157, cg11136562, cg16464322, cg08872742, cg18328933, cg15784615 and cg23282949;
- so that information useful to determine the age of the individual is obtained.
A kit for obtaining information useful to determine the age of an individual, the kit comprising:
- A plurality of primers or probes specific for at least two genomic DNA sequences in a biological sample, wherein the genomic DNA sequences comprise CG loci in the genomic DNA selected from the group consisting of CG locus designation:
- cg27553955, cg12799895, cg21296230, cg04084157, cg25148589, cg17861230, cg03734874, cg18236477, cg27320127, cg07621046, cg14456683, cg21801378, cg00107187, cg05508084, cg07533148, cg03975694, cg19945840, cg00399483, cg06291867, cg24826867, cg06092815, cg19885761, cg24199834, cg04528819, cg13434842, cg02008154, cg25044651, cg02994956, cg20366906, cg27389185, cg14826456, cg12111714, cg12457773, cg11981599, cg00059225, cg06572160, cg08668790, cg12782180, cg20134215, cg13921352, cg13614181, cg23563234, cg00201234, cg21992250, cg00911351, cg19594666, cg15425280, cg06156376, cg19831077, cg27409364, cg15747595, cg02154186, cg20616414, cg10235817, cg16232126, cg24646414, cg17241310, cg10031651, cg23290344, cg06908778, cg02844545, cg14614211, cg25511429, cg15201635, cg19246110, cg06760035, cg13603171, cg13282837, cg20792062, cg02228185, cg13547237, cg09809672, cg01293143, cg05822532, cg07408456, cg08468689, cg27210390, cg01820374, cg17589341, cg19761273, cg03440846, cg08909157, cg11136562, cg16464322, cg08872742, cg18328933, cg15784615 and cg23282949;
- and
- a reagent used in:
- a genomic DNA polymerization process;
- a genomic DNA hybridization process;
- or
- a genomic DNA bisulfite conversion process.
Dependencies are inferred from the claim text.