Methods and Compositions Involving MicroRNA
AssigneeAsuragen, Inc.
InventorsDavid Brown, Rick Conrad, Eric Devroe, Marianna Goldrick, Kerri Keiger, Emmanuel Labourier, Ivonne Moon, Patricia Powers, Jeffrey Shelton, Jaclyn Shingara
Life of this patent
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Abstract
The present invention concerns methods and compositions for isolating, enriching, and/or labeling miRNA molecules and for preparing and using arrays or other detection techniques for miRNA analysis. Moreover, the present invention concerns methods and compositions for generating miRNA profiles and employing such profiles for therapeutic, diagnostic, and prognostic applications.
Claims26
A method for multi-labeling miRNA in a sample comprising:
- a) enriching miRNA with respect to total RNA in the sample;
- b) forming a reaction mixture, under conditions that allows enzyme catalysis, comprising the miRNA with i) an enzyme that catalyzes the addition of di- or tri-phosphate nucleotides to the 3′ end of the miRNA;
- and ii) one or more labeled or unlabeled nucleotides, wherein tailed miRNA molecules are produced;
- c) if unlabeled nucleotides are added to the miRNA, the method further comprising attaching a label to the tailed miRNA molecules.
(canceled)
-14. (canceled)
-23. (canceled)
An miRNA array comprising one or more miRNA probes immobilized on a solid. support, wherein the probes comprise:
- a) an miRNA coding sequence;
- b) an amine attached. to the 5′ or 3′ end. of the probe.
-42. (canceled)
A method for evaluating miRNA in a sample comprising:
- a) isolating miRNA away from other RNA in the sample;
- b) labeling the miRNA.;
- c) hybridizing the miRNA to an miRNA array;
- and,
- d) determining miRNA hybridization to the array.
-147. (canceled)
Dependencies are inferred from the claim text.