Specific alleles important for ethanol tolerance
AssigneesKatholieke Universiteit Leuven, K.U.Leuven R&D, Vib Vzw
InventorsJohan Thevelein, Maria Remedios Foulquié Moreno, Annelies Goovaerts, Steve Swinnen
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Abstract
The present disclosure relates to the identification of a QTL associated with high ethanol tolerance in Saccharomyces spp. More specifically, it relates to specific alleles of MKT1 and APJ1 possibly combined with a specific allele of SWS2 that are important in obtaining a high ethanol tolerance in Saccharomyces spp. It relates further to the use of such alleles in the construction of high ethanol tolerant strains, and the use of these alleles in screening for ethanol tolerance.
Claims13
A method of obtaining ethanol tolerance in a yeast, the method comprising:
- utilizing an inactivated APJ1 allele to obtain ethanol tolerance in the yeast.
A method for screening ethanol resistant yeast, said method comprising:
- identifying a downregulating mutation in the APJ1 gene, and/or
- determining the G30 and/or R453 mutations in the Mkt1p.
Dependencies are inferred from the claim text.