Compositions and methods for glycan sequencing
OwnerJinshan Gao, Jesse L. Beauchampinventors
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Abstract
Compositions and methods directed to a glycan reagent for depolymerization of a glycan having a reducing end, the glycan reagent being represented by the formula MCX, in which M is a glycan coupling group selected from oxylamines and hydrazides; C is a fixed charge group or a basic group having a proton affinity of at least 210 kcal/mol; and X is hydrogen or a free radical initiator chemically coupled to C.
Claims17
A glycan reagent for depolymerization of a glycan having a reducing end, the glycan reagent being represented by the formula MCX, wherein M is a glycan coupling group selected from oxylamines and hydrazides;
- C is a fixed charge group or a basic group having a proton affinity of at least 210 kcal/mol;
- and X is hydrogen or a free radical initiator chemically coupled to C.
A method of identifying subunit connectivity of a glycan having a reducing terminus, comprising:
- conjugating a glycan reagent represented by one of structures (a) through (j) to the reducing terminus of the glycan to form a derivatized glycan
- wherein X is hydrogen, R is hydrogen, R1 and R2 are each independently an alkyl;
- ionizing the derivatized glycan to form an ionized derivatized glycan;
- dissociating the ionized derivatized glycan to form fragment glycan ions;
- and
- analyzing the fragment glycan ions to determine the subunit connectivity of the glycan.
Dependencies are inferred from the claim text.