Adherent cell culture method

2:

• The method of claim 1, wherein each culture space has an equivalent diameter in a range of 100 μm to 5000 μm.

3:

• The method of claim 1, wherein the culture chamber comprises a wall partitioning adjacent culture spaces, wherein the wall has a thickness in a range of 5 μm to 50 μm.

4:

• The method of claim 1, wherein the culture chamber comprises a wall partitioning adjacent culture spaces, and an angle formed between an upper surface and a side surface of the wall is in a range of 90 degrees to 135 degrees.

The method of claim 1, wherein 60% or more of all cultured spheroids have a diameter within a range of 5% below to 5% above an average value of diameters of the spheroids obtained after the culturing.

6:

• The method of claim 1, wherein the spheroids are cancer cells.

The method of claim 1, wherein the culture chamber comprises a resin molded product comprising at least one member selected from the group consisting of an acrylic resin, a polylactic acid, a polyglycolic acid, a styrene resin, an acrylic-styrene copolymer resin, a polycarbonate resin, a polyester resin, a polyvinyl alcohol resin, an ethylene-vinylalcohol copolymer resin, a thermoplastic elastomer, a vinyl chloride resin, and a silicon resin.

8:

• The method of claim 1, wherein the surface of each culture space has been processed by glass processing so that the surface has a water contact angle of 45 degrees or less.

9:

• The method of claim 1, wherein the surface of each culture space has been processed by forming a functional group thereon by plasma treatment so that the surface has a water contact angle of 45 degrees or less.

10:

• The method of claim 1, wherein a polymer having hydrophilic and hydrophobic properties that vary depending on temperature or light is coated on the surface of each culture space.

11:

• The method of claim 1, wherein a hydrophilic polymer chain that inhibits cell adhesion is immobilized on the surface of each culture space.

12:

• The method of claim 1, wherein a phospholipid or a phospholipid-polymer complex is immobilized on the surface of each culture space.

13:

• The method of claim 1, wherein the surface of each culture space has been processed by glass processing so that the surface has a water contact angle of 45 degrees or less, and thereafter a polymer having hydrophilic and hydrophobic properties that vary depending on temperature or light, a hydrophilic polymer chain that inhibits cell adhesion, or a phospholipid or a phospholipid-polymer complex is immobilized on the surface.

14:

• The method of claim 1, wherein the surface of each culture space has been processed by forming a functional group thereon by plasma treatment so that the surface has a water contact angle of 45 degrees or less, and thereafter a polymer having hydrophilic and hydrophobic properties that vary depending on temperature or light, a hydrophilic polymer chain that inhibits cell adhesion, or a phospholipid or a phospholipid-polymer complex is immobilized on the surface.

15:

• The method of claim 2, wherein the culture chamber comprises a wall partitioning adjacent culture spaces, wherein the wall has a thickness in a range of 5 μm to 50 μm.

16:

• The method of claim 2, wherein the spheroids are cancer cells.

17:

• The method of claim 3, wherein the spheroids are cancer cells.

18:

• The method of claim 15, wherein the spheroids are cancer cells.

19:

• The method of claim 5, wherein the spheroids are cancer cells.

20:

• The method of claim 2, wherein 60% or more of all cultured spheroids have a diameter within a range of 5% below to 5% above an average value of diameters of the spheroids obtained after the culturing.