Thermosensitive, bioactive biopolymer and associated method of cell harvesting
AssigneeUmuversidad De Valladolid
InventorsJose Carlos Rodriguez-Cabello, Francisco Javier Arias Vallejo, Matilde AlonsoI Rodrigo, Mercedes Santos Garcia, Maria Pierna Alvarez
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Abstract
The present invention relates to a biopolymer and a cell-harvesting scaffold comprising same, as well as the associated cell-harvesting method that allows said harvesting to be performed in a simple and effective manner by reducing the culture temperature. The present invention also relates to a method for synthesising said biopolymer.
Claims38
A biopolymer comprising peptides A, B and D, with the structure [(D-Bn-Am-Bs)], where
- A has the structure (Ft1G-Ft2), where
- F has the structure X1-X2-X3-X4-X5, where X1 and X4 may be any amino acid except the amino acids proline, lysine, serine and cysteine, X2 is the amino acid proline, X3 is selected from the group consisting of the amino acid glycine and the amino acid alanine and X5 is the amino acid glycine,
- G is a cell-binding sequence,
- t1 and t2 have values of between 8 and 12,
- B has the structure Y1-Y2-Y3-Y4-Y5, where Y1 and Y4 may be any amino acid except the amino acids proline, lysine, serine and cysteine, Y2 is the amino acid proline, Y3 is selected from the group consisting of the amino acid glycine and the amino acid alanine and Y5 is the amino acid glycine,
- D comprises a peptide of 2 to 10 identical or different amino acids selected from the group consisting of lysine, cysteine, serine, asparagine, glutamine, aspartic acid and glutamic acid,
- n has a value of between 10 and 18,
- m has a value of between 1 and 3, and
- s has a value of between 10 and 18.
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Dependencies are inferred from the claim text.