Cell sorting by 3d flow and adhesive rolling
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsSung Young Choi, Rohit N. Karnik, Jeffrey M. Karp
Life of this patent
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Abstract
The present disclosure, among other things, describes cell rolling by 3D flow and adhesive rolling. In some embodiments, a device described herein includes a flow channel dimensioned to permit fluid flow therethrough; at least one 3D structure protruding from at least one surface of the flow channel; and at least one cell adhesion entity coated on at least part of at least one of the 3D structures, which adhesion entity interacts with a target cell brought into contact with the 3D structures by flow of a stream comprising the target cell through the flow channel such that the target cell's trajectory through the flow channel is diverted due to the interaction.
Claims27
A device comprising:
- a flow channel dimensioned to permit a cell stream flow therethrough;
- one or more 3D structures protruding from at least one surface of the flow channel, wherein the 3D structures are arranged and constructed so that at least two sets of streamlines are defined when the cell stream is flowed therethrough;
- at least one cell adhesion entity coated on at least part of the 3D structures, which adhesion entity interacts with a target cell in the cell stream brought into contact with the 3D structures by flow of a stream comprising the target cell through the flow channel such that the target cell's trajectory through the flow channel is diverted due to the interaction.
(canceled)
9-13. (canceled)
The device of claim 13, wherein the 3D structures comprise a plurality of lateral microstructures.
15-16. (canceled)
19-53. (canceled)
A device comprising:
- a flow channel defined by opposing side walls and opposing lower and upper surfaces, wherein a flow channel comprises a longitudinal axis defined along the lower and upper surfaces and parallel to the side walls, a width WS between the side walls, a height HS between the lower and upper surfaces and an inlet for introducing a stream of target and non-target cells into a flow channel;
- and
- a series of lateral microstructures protruding from one or both of the lower and upper surfaces of a flow channel that are coated with a cell adhesion entity, wherein the microstructures comprise a longitudinal axis defined across the width of a flow channel that forms an acute angle αM with the longitudinal axis of a flow channel, a width WM<WS defining a gap between the microstructures and at least one of the side walls and a height HM<HS defining a clearance over or under the microstructures,
- wherein the dimensions of a flow channel and microstructures are such that when a stream of target and non-target cells is flowed along the direction of the longitudinal axis of a flow channel under conditions that permit cell rolling, target cells are diverted laterally from the direction of the longitudinal axis and into the gap between the microstructures and at least one of the side walls as a result of rolling on the microstructures.
61-67. (canceled)
(canceled)
72-98. (canceled)
Dependencies are inferred from the claim text.