Performance-enhanced and temperature-resistant protease variants
AssigneeHenkel AG & Co. KGaA
InventorsHendrik Hellmuth, Marion Merkel, Brian Laufs, Susanne Wieland, Timothy O'Connell, Susanne Tondera, Thomas Weber
Life of this patent
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Abstract
Proteases that comprise an amino acid sequence that is at least 70% identical to the amino acid sequence indicated in SEQ ID NO. 1 over its entire length and that comprise, in the count in accordance with SEQ ID NO. 1, the amino acid substitution R99E or R99D in combination with at least two further amino acid substitutions that are selected from the group consisting of S3T, V4I, and V199I, display very good cleaning performance in particular on blood-containing stains, as well as very good temperature stability.
Claims10
A protease comprising an amino acid sequence that is at least 70% identical to the amino acid sequence indicated in SEQ ID NO. 1 over its entire length and comprises, in the count in accordance with SEQ ID NO. 1, the amino acid substitution R99E or R99D in combination with at least two further amino acid substitutions that are selected from the group consisting of S3T, V4I, and V199I.
A method for manufacturing a protease, comprising the introduction of an amino acid substitution R99E or R99D, in combination with at least two further amino acid substitutions that are selected from the group consisting of S3T, V4I, and V199I, in the count according to SEQ ID NO. 1, into a starting protease that is at least 70% identical to the amino acid sequence indicated in SEQ ID NO. 1 over its entire length.
Dependencies are inferred from the claim text.