Novel mannanase produced from cellulosimicrobium sp. strain hy-13
AssigneeKorea Research Institute of Bioscience and Biotechnology
InventorsHo-Yong Park, Kwang-Hee Son, Do Young Kim, Dong-Ha Shin, Han-Young Cho
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Abstract
The present invention relates to a mannanase produced from Celluosimicrobium sp. strain HY-13 and more particularly to a highly active novel mannanase produced from Cellulosimicrobium sp. strain HY-13 as separated from an invertebrate. The mannanase produced from the said Cellulosimicrobium ap. strain HY-13 can be used in the decomposition of a mannan found in hemicellulose contained in grains and plants and in enhancing the utility value of a hydrolysate, which uses the decomposed mannan, for the transformation of biomass or as an animal feed material.
Claims17
A mannanase characteristically composed of the amino acid sequence of SEQ ID NO:
- 1.
(canceled)
12-13. (canceled)
A method for producing a mannanase comprising the following steps:
- 1) culturing Celluosimicrobium sp. strain HY-13 deposited under the Accession Number of KCTC 11302BP in the medium containing mannan, and obtaining supernatant by centrifuging thereof;
- 2) inducing precipitation of water-soluble proteins by adding a precipitant to the supernatant of step 1);
- 3) obtaining a crude enzyme solution by dialyzing the precipitate of step 2) after eliminating insoluble precipitate from the same;
- and
- 4) purifying mannanase from the crude enzyme solution of step 3).
18-19. (canceled)
Dependencies are inferred from the claim text.