Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
AssigneePromega Corporation
InventorsBrock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
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Abstract
An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
Claims23
-131. (canceled)
A polypeptide comprising a soluble monomeric decapod luciferase polypeptide that is absent of any post translational modifications and catalyzes the emission of luminescence utilizing a coelenterazine as a substrate.
A polypeptide comprising a monomeric decapod luciferase polypeptide that is absent of any post translational modifications and catalyzes the emission of luminescence utilizing a coelenterazine as a substrate.
A polypeptide comprising a soluble monomeric decapod luciferase polypeptide that catalyzes the emission of luminescence utilizing a coelenterazine as a substrate.
Dependencies are inferred from the claim text.