Glucoamylase variants with altered properties
AssigneeDanisco US Inc.
InventorsWolfgang Aehle, Richard R. Bott, Martijn Scheffers, Pieter van Solingen, Casper Vroemen
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Abstract
The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
Claims30
A glucoamylase variant comprising two or more amino acid substitutions corresponding to position 61, 73, 417, 430, 431, 503, 511, 535, 539, or 563 of SEQ ID NO:
- 2, or an equivalent position in a parent glucoamylase, wherein the glucoamylase variant has at least 80% sequence identity with SEQ ID NO:
- 1, 2, 3, 5, 6, 7, 8, or 9.
A glucoamylase variant comprising amino acid substitutions at residues corresponding to positions:
- I43Q/D44C, D44C/G294C, I43Q/G294C, or I43Q/D44C/G294 of SEQ ID NO:
- 2, or an equivalent position in a parent glucoamylase, wherein the glucoamylase variant has at least 80% sequence identity with SEQ ID NO:
- 1, 2, 3, 5, 6, 7, 8, or 9.
Dependencies are inferred from the claim text.