Coryneform Bacterium Transformant and Process for Producing Valine Using the Same

A transformant obtainable by introducing one or more of the following DNAs (a), (b), and (c) into a coryneform bacterium as a host.

• (a) A DNA which encodes acetohydroxy acid synthase derived from Corynebacterium glutamicum and which has a mutation changing the glycine at position 156 to glutamic acid (G156E) in an amino acid sequence encoded by the DNA, or a DNA which hybridizes to a DNA consisting of a complementary base sequence of the DNA under stringent conditions and which encodes a polypeptide having acetohydroxy acid synthase activity.
• (b) A DNA which encodes acetohydroxy acid isomeroreductase derived from Corynebacterium glutamicum and which has mutations changing the serine at position 34 to glycine (S34G), the leucine at position 48 to glutamic acid (L48E), and the arginine at position 49 to phenylalanine (R49F) in an amino acid sequence encoded by the DNA, or a DNA which hybridizes to a DNA consisting of a complementary base sequence of the DNA under stringent conditions and which encodes a polypeptide having acetohydroxy acid isomeroreductase activity.
• (c) A DNA which encodes leucine dehydrogenase derived from Lysinibacillus sphaericus, or a DNA which hybridizes to a DNA consisting of a complementary base sequence of the DNA under stringent conditions and which encodes a polypeptide having leucine dehydrogenase activity.