Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same
AssigneesNovozymes, Inc., Novozymes A/S
InventorNikolaj Spodsberg
Life of this patent
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Abstract
The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims17
1-48. (canceled)
An isolated polypeptide having endoglucanase activity, selected from the group consisting of:
- (a) a polypeptide having at least 85% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, a polypeptide having at least 71% sequence identity to the mature polypeptide of SEQ ID NO:
- 4, a polypeptide having at least 74% sequence identity to the mature polypeptide of SEQ ID NO:
- 6, or a polypeptide having at least 75% sequence identity to the mature polypeptide of SEQ ID NO:
- 8;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, or SEQ ID NO:
- 7, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, or SEQ ID NO:
- 7, or the cDNA sequence thereof;
- (d) a variant of the mature polypeptide of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, or SEQ ID NO:
- 8, comprising a substitution, deletion, and/or insertion at one or more positions;
- and
- (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has endoglucanase activity.
An isolated polypeptide comprising a catalytic domain selected from the group consisting of:
- (a) a catalytic domain having at least 60% sequence identity to the catalytic domain of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, or SEQ ID NO:
- 8;
- (b) a catalytic domain encoded by a polynucleotide having at least 60% sequence identity to the catalytic domain coding sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, or SEQ ID NO:
- 7;
- (c) a variant of a catalytic domain comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the catalytic domain of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, or SEQ ID NO:
- 8;
- and
- (d) a fragment of a catalytic domain of (a), (b), or (c), which has endoglucanase activity.
An isolated polynucleotide encoding a signal peptide comprising or consisting of amino acids 1 to 22 of SEQ ID NO:
- 2, amino acids 1 to 23 of SEQ ID NO:
- 4, amino acids 1 to 20 of SEQ ID NO:
- 6, or amino acids 1 to 19 of SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.