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US 2014/0227722 A1Published application
Multi-biomarker biosensor
AssigneeNortheastern University
InventorsAsanterabi Malima, Ahmed Busnaina, Salome Siavoshi, Sivasubramanian Somu, Cihan Yilmaz, Tiziana Musacchio, Jaydev Upponi, Vladimir Torchilin
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Abstract
Nanosubstrates as biosensors, methods of making such nanosubstrates, and methods of using such nanosubstrates to detect biomarkers are described.
Claims14
A method of preparing a nanosubstrate, comprising:
- providing a first substrate layer;
- depositing an adhesion layer onto a surface of the first substrate layer;
- depositing a second substrate layer onto the adhesion layer;
- depositing a conductive layer onto the second substrate layer;
- depositing an insulating layer onto the conducting layer;
- creating a pattern of nanotrenches or nanowells in the insulating layer by lithography;
- and
- removing the adhesion layer to release the nanosubstrate, the nanosubstrate comprising the second substrate layer, the conductive layer, and the insulating layer.
5 more elements
A method of detecting a biomarker, comprising
- (a) providing a nanosubstrate comprising:
- a first substrate layer;
- a sacrificial layer deposited on the first substrate layer;
- a second substrate layer deposited on the sacrificial layer, the second substrate layer comprising a photoresist material;
- a conductive layer deposited on the second substrate layer;
- and
- an insulating layer deposited on the conductive layer, the insulating layer interrupted by two or more nanotrenches or nanowells having different widths, each width at least about 20 nm;
- two or more nanoparticles deposited in said two or more nanotrenches or nanowells, the nanoparticles belonging to two or more different size classes, wherein the nanoparticles in each size class have a diameter less than or equal to the width of the nanotrench or nanowell in which they are deposited, and wherein the nanoparticles of each size class are covalently bound to a different antibody or antigen binding fragment thereof;
- and
- (b) contacting the nanosubstrate to a biological sample from a subject, the biological sample comprising a biomarker, wherein binding of the biomarker to the antibody or antigen binding fragment thereof results in a detectable signal, thereby detecting the biomarker.
7 more elements
Dependencies are inferred from the claim text.