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US 2014/0227708 A1Published application
Methods and kits used in identifying microrna targets
AssigneeOregon Health & Science University
InventorXiaolu Lim Ang Cambronne
Life of this patent
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Abstract
Described herein are methods and kits used to identify an endogenously expressed target mRNA of a microRNA of interest. The method involves the use of a dominant negative GW182 polypeptide that forms a stable complex with the target mRNA. The method further involves purifying the complex and identifying the target mRNA.
Claims48
A method of identifying a target mRNA of a microRNA of interest, the method comprising:
- contacting the microRNA of interest with a dominant negative GW182 polypeptide within a cell;
- purifying a complex comprising the dominant negative GW182 polypeptide and an endogenously expressed target mRNA of the microRNA of interest;
- and
- identifying the endogenously expressed target mRNA.
1 more element
A kit that facilitates a method of identifying a target mRNA of a microRNA of interest, the kit comprising:
- a first nucleic acid construct that encodes a dominant negative GW182 polypeptide,
- a first reagent used to purify a complex comprising the dominant negative GW182 polypeptide and an endogenously expressed target mRNA of a microRNA of interest;
- and
- a second reagent used to identify the endogenously expressed target mRNA.
1 more element
Dependencies are inferred from the claim text.