Methods and kits for determining dna repair capacity
AssigneeMassachusetts Institute of Technology
InventorsLeona D. Samson, Zachary David Nagel, Carrie Thompson, Isaac Alexander Chaim, Anwaar Ahmad, Anthony L. Forget
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Abstract
In one aspect, the disclosure provides methods, compositions, vectors and kits for determining DNA repair capacity in cells or a subject.
Claims29
A method of determining DNA repair capacity in a cell, the method comprising:
- introducing one or more DNA repair reporter vectors into a cell, and
- determining the capacity of the cell to process the one or more DNA repair reporter vectors thereby determining the DNA repair capacity in the cell.
A method of determining the propensity of a subject to respond to a cancer treatment regimen, the method comprising:
- introducing one or more DNA repair reporter vectors into cells obtained from a subject,
- wherein the one or more DNA repair reporter vectors comprise one or more lesions that are representative of a cancer treatment regimen, and
- determining the capacity of the cells to process the one or more DNA repair reporter vectors thereby determining the propensity of the subject to respond to the cancer treatment regimen.
13-18. (canceled)
A method of determining multiple DNA repair capacities in a cell, the method comprising:
- introducing multiple DNA repair reporter vectors into a cell, and
- determining the capacity of the cell to process the multiple DNA repair reporter vectors thereby determining multiple DNA repair capacities in the cell.
31-35. (canceled)
A kit for determining the propensity of a subject to respond to a cancer treatment regimen comprising one or more DNA repair reporter vectors, wherein the one or more DNA repair reporter vectors comprise lesions representative of a cancer treatment regimen, and instructions for use of the one or more DNA repair reporter vectors.
A kit for determining the susceptibility of a subject to an environmental condition comprising one or more DNA repair reporter vectors, wherein the one or more DNA repair reporter vectors comprise lesions representative of an environmental condition, and instructions for use of the one or more DNA repair reporter vectors.
38-49. (canceled)
Dependencies are inferred from the claim text.