Back to results
US 2014/0227700 A1Published application
Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders
AssigneeEpigenomics AG
InventorsAndrew Z. Sledziewski, Catherine E. Lofton-Day, Reimo Tetzner, Juergen Distler, Fabian Model, Shannon Payne, Dimo Dietrich
Life of this patent
Estimating the term…
Abstract
The invention provides methods, nucleic acids and kits for detecting prostate cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of said disorder, thereby enabling the improved diagnosis and treatment of patients.
Claims5
A method for detecting prostate cell proliferative disorders in a subject comprising the steps of:
- a) treating genomic DNA which is derived from a biological sample comprising tumor cells with bisulfite so that unmethylated cytosine bases are converted to uracil while methylated cytosine bases remain unconverted, wherein the genomic DNA comprises a sequence of at least 16 contiguous nucleotides of SEQ ID NO:
- 1 and the 16 contiguous nucleotides comprise at least one CpG dinucleotide;
- b) detecting unconverted cytosine bases in the at least 16 contiguous nucleic acids of SEQ ID NO 1 based on their base pairing properties;
- c) detecting a prostate cell proliferative disorder based on the presence of at least one unconverted cytosine comprised by a CpG dinucleotide in the at least 16 contiguous nucleic acids of SEQ ID NO 1.
1 more element
Dependencies are inferred from the claim text.