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AssigneeEvocatal GmbH
InventorsThomas Drepper, Karl-Erich Jaeger, Janko Potzkei
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To investigate protein-protein interactions, protein foldings and protein localization and also in the secretion of proteins, in vivo reporter proteins are used in biotechnology and in basic research. In order to be able to utilize fluorescence reporters as markers for secretion processes, FMN-binding fluorescence proteins (FbFP) have been developed by us for the first time. The new fluorescence markers can be expressed like GFP in various bacteria. The binding of the chromophore FMN produces a cyan-green fluorescent protein which can be detected in vivo using all customary spectroscopic and microscopic methods. In contrast to GFP, this protein can also surprisingly be secreted via the Sec route and be converted to the fluorescence-active state in the periplasma.
(canceled)
The method according to claim 12, wherein at least one cysteine in the LOV domain of the fluorescent protein is replaced by alanine.
The method according to claim 12, wherein the LOV domain of the fluorescent protein comprises at least one further point mutation in addition to the exchange of the at least one cysteine.
The method according to claim 12, wherein the fluorescent protein comprising the LOV domain that
The method according to claim 12, wherein the fluorescent protein comprises a size between ≧16 kDa and ≦19 kDA.
The method according to claim 12, wherein the fluorescent protein comprises an excitation wavelength between ≧430 nm and ≦470 nm.
The method according to claim 12, wherein the fluorescent protein comprises an emission maximum between ≧470 nm and ≦520 nm.
The method according to claim 12, wherein the fluorescent protein is being expressed or co-expressed in a host cell, and being secreted into the periplasm and/or into an extracellular media.
The method according to claim 12, wherein the fluorescent protein comprises a signal sequence at its N-terminus.
A method for labelling an antibody expressed in an organism, wherein the method comprises
Dependencies are inferred from the claim text.