Stabilisation and isolation of extracellular nucleic acids
AssigneeQiagen GmbH
InventorsMartin Horlitz, Anabelle Schubert, Markus Sprenger-Haussels
Life of this patent
Estimating the term…
Abstract
The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample using an apoptosis inhibitor, preferably a caspase inhibitor, a hypertonic agent and/or a compound according to formula (1) as defined in the claims.
Claims21
A method for stabilizing an extracellular nucleic acid population comprised in a cell-containing sample by contacting a sample with a caspase inhibitor.
A composition suitable for stabilizing the extracellular nucleic acid population in a biological sample, preferably a blood sample, comprising
- a) at least one caspase inhibitor,
- and comprising at least one further compound selected from b), c) and d), wherein
- b) is at least one hypertonic agent suitable for stabilizing cells comprised in the sample, preferably is at least one hydroxylated organic compound;
- c) is at least one compound according to formula 1,
- wherein R1 is a hydrogen residue or an alkyl residue, preferably a C1-C5 alkyl residue, more preferred a methyl residue, R2 and R3 are identical or different hydrocarbon residues with a length of the carbon chain of 1-20 atoms arranged in a linear or branched manner, and R4 is an oxygen, sulphur or selenium residue;
- d) is at least one anticoagulant, preferably a chelating agent.
Dependencies are inferred from the claim text.