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US 2014/0227685 A1Published application
Rapid freeze-quench device and methods of use thereof
AssigneeYeda Research and Devlopment Co.Ltd
InventorsDaniella Goldfarb, Royi Kaufmann
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Abstract
This invention provides mixing, reaction and ejection devices for small volume samples. This invention also provides cooling devices, sample preparation apparatuses and sample preparation methods for studying chemical and biological reactions. The mixing, reaction and ejection devices of this invention allow ejection of small volume liquid samples from a microfluidic channel. Cooling devices of the invention enable serial cooling and collection of samples in an efficient and accurate manner. This invention also provides apparatuses comprising the mixing, reaction and ejection devices and cooling devices. This invention provides methods of using the novel devices and apparatuses.
Claims46
A microfluidic device comprising:
- a microfluidic channel comprising a first mixing part and a second reaction part, wherein said channel further comprises a first inlet and a second inlet;
- a product outlet, wherein said product outlet is attached to said microfluidic channel;
- a gas compartment surrounding at least a portion of said product outlet such that said gas compartment is coaxial with said product outlet, wherein said gas compartment is attached to a gas inlet channel, and wherein said gas compartment comprises a gas outlet proximal to said product outlet;
- wherein upon introduction of a first material from said first inlet and a second material from said second inlet, said first material and said second material are mixed within said first mixing part, and wherein at least a portion of said materials react within said second reaction part and wherein upon ejection of mixed and at least partially reacted materials from said product outlet, a gas from said gas compartment is mixed with said materials thus facilitating ejection of said materials from said product outlet.
1 more element
An apparatus for sample preparation, said apparatus comprising:
- a microfluidic device comprising:
- a microfluidic channel comprising a first mixing part and a second reaction part, wherein said channel further comprises a first inlet and a second inlet;
- a product outlet, wherein said product outlet is attached to said microfluidic channel;
- a gas compartment surrounding at least a portion of said product outlet such that said gas compartment is coaxial with said product outlet, wherein said gas compartment is attached to a gas inlet channel, and wherein said gas compartment comprises a gas outlet proximal to said product outlet;
- wherein upon introduction of a first material from said first inlet and a second material from said second inlet, said first material and said second material are mixed within said first mixing part, and wherein at least a portion of said materials react within said second reaction part and wherein upon ejection of mixed and at least partially reacted materials from said product outlet, a gas from said gas compartment is mixed with said materials thus facilitating ejection of said materials from said product outlet;
- a cooling device comprising:
- a translation element comprising a surface;
- and
- a cooling means;
- wherein said translation element is cooled by said cooling means;
- wherein said product outlet of said microfluidic device is placed in proximity to said cooling device such that material can be transferred from said microfluidic device onto different areas on said surface of said translation element.
8 more elements
A method of preparation of samples for analysis, said method comprising:
- a. providing an apparatus for analysis, said apparatus comprising:
- a microfluidic device comprising:
- a microfluidic channel comprising a first mixing part and a second reaction part, wherein said channel further comprises a first inlet and a second inlet;
- a product outlet, wherein said product outlet is attached to said microfluidic channel;
- a gas compartment surrounding at least a portion of said product outlet such that said gas compartment is coaxial with said product outlet, wherein said gas compartment is attached to a gas inlet channel, and wherein said gas compartment comprises a gas outlet proximal to said product outlet;
- wherein upon introduction of a first material from said first inlet and a second material from said second inlet, said first material and said second material are mixed within said first mixing part, and wherein at least a portion of said materials react within said second reaction part and wherein upon ejection of mixed and at least partially reacted materials from said product outlet, a gas from said gas compartment is mixed with said materials thus facilitating ejection of said materials from said product outlet;
- a cooling device comprising:
- a translation element comprising a surface;
- and
- a cooling means;
- wherein said translation element is cooled by said cooling means;
- wherein said product outlet of said microfluidic device is placed in proximity to said cooling device such that material can be transferred from said microfluidic device onto different areas on said surface of said translation element.
- b. cooling said translation element using said cooling means;
- c. translating said surface of said translation element such that said top surface of said translation element remains at a constant vertical distance from said microfluidic device;
- d. injecting a first reactant solution from said first inlet and a second reactant solution from said second inlet such that said first reactant and said second reactant get into contact at said microfluidic channel and wherein said reactants are mixed within said mixing part and wherein said reactants at least partially react within said reaction part;
- e. ejecting samples comprising at least partially reacted materials in a serial manner from said product outlet onto different areas of said surface of said translation element, wherein upon ejecting, the product solution is mixed with gas ejected from said gas compartment thus facilitating ejection of said product solution.
13 more elements
Dependencies are inferred from the claim text.