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US 2014/0227683 A1Published application
Probe with multiple target region specificity and of tripartite character
AssigneeEpistem Limited
InventorBen Cobb
Life of this patent
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Abstract
Probes and methods are described for detecting polymorphisms, including short tandem repeats, in a target nucleotide sequence. The probes include first and second regions separated by a linker sequence, with the first and second regions having discrete melting temperatures with their respective target sequences. In a first embodiment, the first and second regions are both reporter sequences; in a second embodiment, one region is an anchor sequence while the other is a reporter sequence.
Claims27
A nucleic acid probe comprising:
- a first nucleic acid sequence being complementary to a first target nucleic acid sequence;
- a second nucleic acid sequence being complementary to a second target nucleic acid sequence;
- and a linker nucleic acid sequence joining the first and second nucleic acid sequences;
- wherein the linker separates the two first and second sequences such that the melting temperature of the first sequence annealed to the first target nucleic acid sequence and of the second sequence annealed to the second target nucleic acid sequence are discrete.
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A nucleic acid probe comprising:
- a first anchor nucleic acid sequence being complementary to a first target nucleic acid sequence;
- a second reporter nucleic acid sequence being complementary to a second target nucleic acid sequence;
- and a linker nucleic acid sequence joining the first and second nucleic acid sequences;
- wherein the linker separates the first and second sequences such that the melting temperatures of the first and second sequences hybridised to their respective target sequences is discrete, and where the melting temperature of the anchor sequence annealed to the first target nucleic acid sequence is higher than that of the reporter sequence annealed to the second target nucleic acid sequence;
- and wherein each reporter sequence comprises at least one detectable label.
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Dependencies are inferred from the claim text.