Compositions, methods and kits to detect adenovirus nucleic acids
AssigneeGEN-PROBE PRODESSE, INC., c/o GEN-PROBE INCORPORATED
InventorsEmily Ziegler, Jessica Townsend
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Abstract
The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Adenovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers.
Claims23
1-15. (canceled)
A composition for use in an Adenovirus target nucleic acid amplification assay comprising at least three amplification oligomers capable of stably hybridizing to Adenovirus target nucleic acid to generate an amplicon during an amplification reaction, wherein the at least three amplification oligomers are
- (a) a first amplification oligomer consisting of a target hybridizing sequence as set forth in SEQ ID NO:27,
- (b) a second amplification oligomer consisting of a target hybridizing sequence as set forth in SEQ ID NO:28, and
- (c) a third amplification oligomer consisting of target hybridizing sequences as set forth in (i) SEQ ID NO:25 and/or SEQ ID NO:26, (ii) SEQ ID NO:33 and/or SEQ ID NO:34, or (iii) SEQ ID NO:31 and/or SEQ ID NO:26.
(canceled)
19-25. (canceled)
A method for specifically detecting an Adenovirus target nucleic acid in a sample comprising the steps of:
- (a) isolating an Adenovirus target nucleic acid from a sample;
- (b) providing reagents and conditions sufficient for generating an amplicon from the Adenovirus target nucleic acid using amplification oligomers;
- (c) providing at least one labelled detection probe oligonucleotide for detecting the amplicon, wherein independently each of the detection probe oligonucleotides is configured to specifically hybridize to a portion of a region corresponding to nucleotides 1 to 99 of SEQ ID NO:47 or the complementary nucleic acid strand thereof;
- and
- (d) providing conditions for detecting said amplicon and determining whether an Adenovirus target nucleic acid is present in said sample.
A reaction mixture for use in an Adenovirus detection assay, wherein the reaction mixture comprises at least one labelled detection probe oligonucleotide for detecting an amplicon, wherein independently each of the detection probe oligonucleotides is configured to specifically hybridize to a portion of a region corresponding to nucleotides 1 to 99 of SEQ ID NO:47 or the complementary nucleic acid strand thereof.
Dependencies are inferred from the claim text.