Polypeptides Having Alpha-Glucosidase Activity And Polynucleotides Encoding Same
AssigneeNovozymes, Inc.
InventorsSuzanne Otani, Haiyan Ge, Paul Harris, Debbie Yaver, Alexander Blinkovsky
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Abstract
The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Claims20
An isolated polypeptide having alpha-glucosidase activity, selected from the group consisting of:
- (a) a polypeptide having an amino acid sequence which has at least 75% identity with amino acids 15 to 881 of SEQ ID NO:
- 2 or amino acids 30 to 967 of SEQ ID NO:
- 4, or at least 85% identity with amino acids 20 to 988 of SEQ ID NO:
- 6;
- (b) a polypeptide which is encoded by a polynucleotide which hybridizes under medium stringency conditions with (i) nucleotides 43 to 2643 of SEQ ID NO:
- 1 or nucleotides 146 to 3013 of SEQ ID NO:
- 3, (ii) the cDNA sequence contained in nucleotides 43 to 2643 of SEQ ID NO:
- 1 or nucleotides 146 to 3013 of SEQ ID NO:
- 3, or (iii) a complementary strand of (i) or (ii), or under high stringency conditions with (i) nucleotides 58 to 3164 of SEQ ID NO:
- 5, (ii) the cDNA sequence contained in nucleotides 58 to 3164 of SEQ ID NO:
- 5, or (iii) a complementary strand of (i) or (ii);
- and
- (c) a variant comprising a conservative substitution, deletion, and/or insertion of one or more amino acids of amino acids 15 to 881 of SEQ ID NO:
- 2, amino acids 30 to 967 of SEQ ID NO:
- 4, or amino acids 20 to 988 of SEQ ID NO:
- 6.
A nucleic acid construct comprising a gene encoding a protein operably linked to a nucleotide sequence encoding a signal peptide consisting of nucleotides 1 to 42 of SEQ ID NO:
- 1, nucleotides 1 to 145 of SEQ ID NO:
- 3, or nucleotides 1 to 57 of SEQ ID NO:
- 5, wherein the gene is foreign to the nucleotide sequence.
Dependencies are inferred from the claim text.