Methods of manufacturing bioactive gels from extracellular matrix material
OwnerKimberly A. Kentner, Katherine A. Stuart +1inventors
InventorsKimberly A. Kentner, Katherine A. Stuart, Abram D. Janis
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Abstract
The present invention is directed to methods of manufacturing bioactive gels from ECM material, i.e., gels which retain bioactivity, and can serve as scaffolds for preclinical and clinical tissue engineering and regenerative medicine approaches to tissue reconstruction. The manufacturing methods take advantage of a new recognition that bioactive gels from ECM material can be created by digesting particularized ECM material in an alkaline environment and neutralizing to provide bioactive gels.
Claims5
A method of manufacturing a bioactive gel from an extracellular matrix material comprising (a) providing an ECM material from the group consisting of small intestine submucosa (SIS), urinary bladder submucosa (UBS), urinary bladder matrix (UBM) and liver basement membrane (LBM), (b) particularizing the ECM to a particle size in the range of about 1 μm to about 100 μm, (e) solubilizing concentrations in the range of about 0.5 to 11% w/v of particularized powder in NaOH in the range of 0.1 to 1.0 M for periods of time ranging from about 1 hour to about 48 hours, (d) neutralizing the solubilized ECM prepared in step (c) with equimolar hydrochloric acid ranging from 0.1M to 1.0M to form the gel, and (e) optionally, freeze drying the neutralized solubilized ECM prepared in step (d), (f) lyophilizing the freeze dried ECM prepared in step (e) and (f) reconstituting the lyophilized gel in water.
Dependencies are inferred from the claim text.