Regenerative tissue grafts and methods of making same
AssigneesThe Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., The Government of the United States, as represented by the Secretary of the Department of, The United States Government, AS Represented By the Secretary of the Army, U.S.A.
InventorsWesley M. Jackson, Leon J. Nesti, Rocky S. Tuan
Life of this patent
Estimating the term…
Abstract
A graft containing a scaffold that includes a matrix in which are positioned mesenchymal progenitor cells (MPCs) has the capacity to substantially improve wound healing, including wounds resulting from injury to nerve, bone and vascular tissue. MPCs can be harvested from debrided muscle tissue following orthopaedic trauma. The traumatized muscle-derived progenitor cells are a readily available autologous cell source that can be utilized to effect or improve wound healing in a variety of therapeutic settings and vehicles.
Claims28
A graft comprising a scaffold and a therapeutic component;
- the scaffold comprising a porous matrix of non-aligned fibers forming interstices, and wherein the therapeutic component consists of a cellular component and a non-cellular component, and wherein the majority of the cellular component is mesenchymal progenitor cells.
A graft conduit comprising:
- a) a core of aligned fibers;
- and
- b) a sheath of non-aligned fibers forming interstices and surrounding the core, wherein the interstices consist of a cellular component and a non-cellular component, and wherein the majority of the cellular component is mesenchymal progenitor cells.
A method of isolating mesenchymal progenitor cells comprising:
- a) structural degradation of a muscle sample from a wound;
- b) suspension of the sample in a digestion medium;
- c) culturing and/or incubating the sample;
- and
- d) isolating mesenchymal progenitor cells.
A method for isolating mesenchymal progenitor cells comprising:
- a) removal of unhealthy tissue from muscle in a wound;
- b) isolation of a sample of the muscle;
- washing the muscle sample;
- c) structural degradation of the muscle to create a tissue suspension;
- d) washing the tissue suspension of the sample in a digestion medium;
- e) culturing and/or incubating the sample;
- and
- f) isolating a cell population, the majority of which are mesenchymal progenitor cells.
A method for isolating mesenchymal progenitor cells comprising:
- a) suspending a muscle sample from a wound in digestion medium;
- b) mincing the sample to an average particle size of about 5 mm3 or less;
- c) transferring the minced product to digestion medium;
- d) digesting the sample;
- e) filtering the cultured sample;
- f) centrifuging the filtered cultured sample;
- g) collecting and resuspending a pellet from the centrifugation step;
- and
- h) collecting mesenchymal progenitor cells from the resuspension.
A method of treating a patient suffering an injury to tissue and requiring restoration and/or regeneration of the tissue comprising applying to the injury a graft comprising a porous matrix of non-aligned fibers forming interstices, the interstices consisting of a cellular component and a non-cellular component, and wherein the majority of the cellular component is mesenchymal progenitor cells.
A method of treating a patient suffering a nerve injury producing a gap in the nerve comprising connecting a proximal nerve stump to a distal nerve stump with a nerve graft conduit comprising:
- a) a core of aligned fibers;
- and
- b) a sheath of non-aligned fibers forming interstices and surrounding the core, wherein the interstices consist of a cellular component and a non-cellular component, and wherein the majority of the cellular component is mesenchymal progenitor cells.
A graft comprising:
- a) a porous matrix of non-aligned fibers forming interstices;
- b) the interstices consisting of a cellular component and a non-cellular component, and wherein the majority of the cellular component is mesenchymal progenitor cells,
- c) a conduit internal to the matrix;
- and
- d) the interior of the conduit is in fluid communication with the matrix.
Dependencies are inferred from the claim text.