Rabies virus like particle production in plants

A method of producing a rabies virus like particle (VLP) in a plant comprising,

• a) introducing a nucleic acid comprising a regulatory region active in the plant operatively linked to a nucleotide sequence encoding a native rabies glycoprotein (G), into the plant, or portion of the plant,
• b) incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acid, thereby producing the rabies VLP,
• c) harvesting the plant, and
• d) extracting the VLPs, wherein the VLPs range in size from 40-300 nm and,
• wherein the VLP comprises a lipid obtained from a plasma membrane of the plant.

A method of producing a rabies virus like particle (VLP) in a plant comprising,

• a) providing a plant or portion of the plant comprising a nucleic acid comprising a regulatory region active in the plant operatively linked to a nucleotide sequence encoding one or more native rabies glycoprotein (G),
• b) incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acid, thereby producing the rabies VLP,
• c) harvesting the plant, and
• d) extracting the VLPs, wherein the VLPs range in size from 40-300 nm, and
• wherein the VLP comprises a lipid obtained from a plasma membrane of the plant.