Epstein-barr-virus vaccine

A method for generating a vaccine particle, said method comprising the steps of:

• (a) transfecting a cell with a modified EBV genome, wherein said modified EBV genome in comparison to a wildtype EBV genome:
• i. lacks one or more sequences that are required for the packaging of said wildtype EBV genome, lacks one or more sequences encoding EBV polypeptides required for said packaging, or comprises one or more sequences encoding EBV polypeptides whose packaging capacity is disabled,
• ii. lacks one or more sequences encoding EBV polypeptides that are required for B-cell transformation or comprises one or more sequences encoding EBV polypeptides whose B-cell transformation capacity is disabled;
• and
• iii. lacks one or more sequences encoding EBV polypeptides that are required for inducing replication of an EBV and/or comprises one or more sequences encoding EBV polypeptides whose capacity for inducing EBV replication is disabled;
• (b) culturing the cell obtained in step (a) under conditions that allow expression of said modified EBV genome;
• (c) inducing the replicative phase of EBV;
• and
• (d) isolating said vaccine particle.