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US 2014/0227244 A1Published application
Coupling endonucleases with end-processing enzymes drives high efficiency gene disruption
OwnerAndrew M. Scharenberg, Michael T. Certo +1inventors
InventorsAndrew M. Scharenberg, Michael T. Certo, Kamila Sabina Gwiazda
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Abstract
The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.
Claims16
A method of increasing the mutagenic activity of an endonuclease comprising:
- providing the endonuclease concurrently with an end-processing enzyme.
A polypeptide comprising an endonuclease domain, and end-processing domain.
A method of treating a disease in a subject comprising:
- contacting one or more cells of the subject with one or more polypeptides having endonuclease activity and one or more polypeptides having end-processing activity,
- wherein the one or more polypeptides having endonuclease activity bind to a portion of a nucleotide sequence within the cells encoding a gene involved in the disease or a regulatory sequence thereof.
Dependencies are inferred from the claim text.