Pegylated Interleukin-10
AssigneeMerck Sharp & Dohme Corp.
InventorsSeoju Lee, David C. Wylie, Susan Cannon-Carlson
Life of this patent
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Abstract
Interleukin-10 (IL-10) conjugated via a linker to one or more polyethylene glycol (PEG) molecules at a single amino acid residue of the IL-10, and a method for preparing the same, are provided. The method produces a stable mono-pegylated IL-10, which retains IL-10 activity, where pegylation is selective for the N-terminus on one subunit of IL-10 with little or no formation of monomeric IL-10. The method also provides a substantially homogenous population of mono-PEG-IL-10.
Claims18
-20. (canceled)
A mono-pegylated interleukin-10 (mono-PEG-IL-10) comprising one or more polyethylene glycol (PEG) molecules covalently attached via a linker to a single amino acid residue of a single subunit of IL-10,
- wherein said amino acid residue is the N-terminal amino acid residue,
- wherein said linker is attached to the alpha amino group of the N-terminal amino acid residue, and
- wherein the total molecular mass of the one or more PEG molecules and the linker is from 3,000 daltons to 60,000 daltons.
Dependencies are inferred from the claim text.