Optimization of Vectors for Effective Delivery and Expression of Genetic Content
AssigneeThermo Fisher Scientific Biosciences Inc.
InventorJohn Wakefield
Life of this patent
Estimating the term…
Abstract
Lentiviral vectors, plates, kits and methods are provided that permit improved efficiency for selection of a promoter sequence for use in a lentiviral application. Through various embodiments of the present invention, a researcher may first evaluate and then choose in a modular fashion, a vector comprising a promoter that is effective for expression in a particular cell line or type.
Claims26
A plate for selection of a promoter sequence, wherein the plate comprises a first row of a plurality of loci and a second row of a plurality of loci, wherein at least two of the plurality of loci within the first row each comprise a first lentiviral vector comprising:
- (a) a promoter sequence selected from the group consisting of sequences for human CMV, mouse CMV, human EF1α, mouse EF1α, CAG, mouse PGK, and UBC;
- and
- (b) a reporter sequence;
- and
- at least two of the plurality of loci within the second row each comprise a second lentiviral vector, wherein the second lentiviral vector is the same as the first lentiviral vector except that it comprises a different promoter sequence selected from the group consisting of sequences for human CMV, mouse CMV, human EF1α, mouse EF1α, CAG, mouse PGK, and UBC.
A lentiviral vector comprising:
- (a) a promoter sequence selected from the group consisting of sequences for human CMV, mouse CMV, human EF1α, mouse EF1α, CAG, mouse PGK, and UBC;
- and
- (b) a reporter sequence.
A plate for selection of a promoter sequence, wherein the plate comprises a first row of a plurality of loci and a second row of a plurality of loci, wherein at least two of the plurality of loci within the first row each comprise a first lentiviral vector comprising:
- (a) a first promoter sequence;
- and
- (b) a reporter sequence;
- and
- at least two of the plurality of loci within the second row each comprise a second lentiviral vector, wherein the second lentiviral vector is the same as the first lentiviral vector except that it does not comprise said first promoter sequence and comprises a second promoter sequence that is different from the first promoter sequence.
Dependencies are inferred from the claim text.