Method of treating hyperproliferation of cells using a nucleolin specific aptamer
AssigneePostech Academy-Industry Foundation
InventorsJUNG HWAN LEE, SOON HAG KIM, MI-JIN KWON, HYUNGU KANG, SUNG HO RYU, JONG IN KIM, YOUNDONG KIM, YOUNG CHAN CHAE, SUNG KEY JANG, JONG HUN IM, SUN HAK LEE, HYE JUNG LEE, EUN JUNG JANG, KI SEOK KIM
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Abstract
Improved G-rich oligonucleotide (GRO) aptamers specific to nucleolin, a method of preparing the aptamers, and a use of the aptamers for diagnosing and/or treating a nucleolin-associated disease, are provided.
Claims9
A method of treating a cancer, comprising administering a nucleolin-specific aptamer a subject in need of treating a cancer, wherein the nucleolin-specific aptamer has the nucleotide sequence of SEQ ID NO:
- 3, and one or more thymidines(T) present in the nucleotide sequence are independently substituted with a modified pyrimidine nucleoside, and wherein the modified pyrimidine nucleoside is deoxyuridine(dU), deoxycytidine(dC), uridine(U), or cytidine(C) having a hydrophobic group at 5-position:
- (SEQ ID NO:
- 3)
- NGGTGGTGGTGGTTGTGGTGGTGGTGGN
- wherein each N is absent or 1 to 20 nucleosides, which is independently selected from the group consisting of adenosine(A), thymidine(T)/uridine(U), cytidine(C), and guanosine(G).
A method of inhibiting nucleolin, comprising administering a nucleolin-specific aptamer to a subject in need of inhibiting nucleolin or a sample comprising nucleolin-expressing cells, wherein the nucleolin-specific aptamer has the nucleotide sequence of SEQ ID NO:
- 3, and one or more thymidines(T) present in the nucleotide sequence are independently substituted with a modified pyrimidine nucleoside, and wherein the modified pyrimidine nucleoside is deoxyuridine(dU), deoxycytidine(dC), uridine(U), or cytidine(C) having a hydrophobic group at 5-position:
- (SEQ ID NO:
- 3)
- NGGTGGTGGTGGTTGTGGTGGTGGTGGN
- wherein each N is absent or 1 to 20 nucleosides, which is independently selected from the group consisting of adenosine(A), thymidine(T)/uridine(U), cytidine(C), and guanosine(G).
A method of inhibiting hyperproliferation of cell cased by nucleolin, comprising administering a nucleolin-specific aptamer to a subject in need of inhibiting hyperproliferation of cell cased by nucleolin or a sample comprising nucleolin-expressing cells, wherein the nucleolin-specific aptamer has the nucleotide sequence of SEQ ID NO:
- 3, and one or more thymidines(T) present in the nucleotide sequence are independently substituted with a modified pyrimidine nucleoside, and wherein the modified pyrimidine nucleoside is deoxyuridine(dU), deoxycytidine(dC), uridine(U), or cytidine(C) having a hydrophobic group at 5-position:
- (SEQ ID NO:
- 3)
- NGGTGGTGGTGGTTGTGGTGGTGGTGGN
- wherein each N is absent or 1 to 20 nucleosides, which is independently selected from the group consisting of adenosine(A), thymidine(T)/uridine(U), cytidine(C), and guanosine(G).
Dependencies are inferred from the claim text.