Compositions and methods for the treatment of sepsis and other disorders involving phospholipase a2 induction
OwnerMaw-Shung Liuinventor
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Abstract
The present invention provides antisense oligomers to PLA2 to inhibit PLA2 protein expression and enzyme activity, and to treat diseases and disorders associated with induced expression of PLA2. In particular, the invention provides for the simultaneous inhibition of cPLA2 and sPLA2.
Claims20
An antisense oligonucleotide of 15 to 25 bases comprising a sequence selected from the group consisting of SEQ ID NO:1 (5′-GTCTTCATGGTAAGAGTT-3′), SEQ ID NO:2 (5′-TCTTACCAAAGATCATGAT-3′), SEQ ID NO:3 (5′-GGACTCTTACCACAG-3′), SEQ ID NO:4 (5′-CTCACCGATCCGTTGCAT-3′), SEQ ID NO:5 (5′-CCTCACCGATCCGTTGCAT-3′), SEQ ID NO:6 (5′-TTTATTCAGAAGAGA-3′), SEQ ID NO:7 (5′-GCTCCACCTGGAAAT-3′), SEQ ID NO:8 (5′-GTGCTCCACCTGGAAA-3′), SEQ ID NO:9 (5′-GAATACTGGTGCTCCAC-3′), SEQ ID NO:10 (5′-TTTATCACCTGCAAATAG-3′), SEQ ID NO:11 (5′-CCTCAATGCCTCTAGCTTTC-3′), SEQ ID NO:12 (5′-TCTATAAATGACATTTTGG-3′), SEQ ID NO:14 (5′-CATCCTTGGGGGATC-3′), SEQ ID NO:15 (5′-GTGCCACATCCACGT-3′), SEQ ID NO:17 (5′-AGAATCCCACCATGGC-3′). SEQ ID NO:19 (5′-TTCCCAGCACGTCCTTCTC-3′), SEQ ID NO:20 (5′-GGGATACGGCAGGTT-3′) or SEQ ID NO:21 (5′-AGGATCAATCTTTGG-3′).
The antisense oligonucleotide 1, wherein said antisense oligonucleotide is 16-25 bases.
The antisense oligonucleotide 1, wherein said antisense oligonucleotide is 15-20 bases.
A method of reducing phospholipase A2 expression in a cell comprising contacting said cell with two or more antisense oligonucleotides of 15-25 bases and comprising a sequence selected from the group consisting of SEQ ID NO:1 (5′-GTCTTCATGGTAAGAGTT-3′), SEQ ID NO:2 (5′-TCTTACCAAAGATCATGAT-3′), SEQ ID NO:3 (5′-GGACTCTTACCACAG-3′), SEQ ID NO:4 (5′-CTCACCGATCCGTTGCAT-3′), SEQ ID NO:5 (5′-CCTCACCGATCCGTTGCAT-3′), SEQ ID NO:6 (5′-TTTATTCAGAAGAGA-3′), SEQ ID NO:7 (5′-GCTCCACCTGGAAAT-3′), SEQ ID NO:8 (5′-GTGCTCCACCTGGAAA-3′), SEQ ID NO:9 (5′-GAATACTGGTGCTCCAC-3′), SEQ ID NO:10 (5′-TTTATCACCTGCAAATAG-3′), SEQ ID NO:11 (5′-CCTCAATGCCTCTAGCTTTC-3′), SEQ ID NO:12 (5′-TCTATAAATGACATTTTGG-3′), SEQ ID NO:14 (5′-CATCCTTGGGGGATC-3′), SEQ ID NO:15 (5′-GTGCCACATCCACGT-3′), SEQ ID NO:17 (5′-AGAATCCCACCATGGC-3′), SEQ ID NO:19 (5′-TTCCCAGCACGTCCTTCTC-3′), SEQ ID NO:20 (5′-GGGATACGGCAGGTT-3′) or SEQ ID NO:21 (5′-AGGATCAATCTTTGG-3′).
Dependencies are inferred from the claim text.