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US 2014/0213501 A1Published application
Lipoprotein complexes and manufacturing and uses thereof
AssigneeCerenis Therapeutics Holding SA
InventorsJean-Louis DASSEUX, Rose ACKERMANN, Daniela Carmen ONICIU
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Abstract
The present disclosure relates to lipoprotein complexes and lipoprotein populations and their use in the treatment and/or prevention of dyslipidemic diseases, disorders, and/or conditions. The disclosure further relates to recombinant expression of apolipoproteins, purification of apolipoproteins, and production of lipoprotein complexes using thermal cycling-based methods.
Claims20
1-25. (canceled)
A method for preparing lipoprotein complexes, comprising:
- (a) cooling a starting suspension comprising a lipid component and a protein component from a temperature in a first temperature range to a temperature in a second temperature range, wherein said lipid component consists essentially of particles of lipids and wherein said protein component consists essentially of lipid-binding peptides and/or lipid-binding proteins;
- (b) heating the cooled suspension of (a) from a temperature in said second temperature range to a temperature in said first temperature range;
- (c) cooling said heated suspension of (b) from a temperature in said first temperature range to a temperature in said second temperature range;
- and
- (d) repeating steps (b) and (c) at least once, until at least 80% of the protein component is incorporated into lipoprotein complexes, thereby forming lipoprotein complexes.
2 more elements
28-29. (canceled)
Dependencies are inferred from the claim text.